Part:BBa_K1073034:Design
Inducible ampR expression combined with LasR, RhlI and aeBlue expression cassettes
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1306
Illegal NheI site found at 1329
Illegal NheI site found at 2988
Illegal NheI site found at 3011 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2830
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1683
Illegal AgeI site found at 1880 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
no considerations.
Source
The parts for this construct were derived from the 2013 distribution kit and the iGEM Team Uppsala.
References
[Fisher et al., 1978]
Fisher et al. Kinetic studies on the inactivation of Escherichia coli RTEM β-lactamase by clavulanic acid. Biochemistry, 1978, 17 (11), pp 2180–2184
[Medina et al., 2003]
Medina G., et al., Mechanism of Pseudomonas aeruginosa RhlR transcriptional regulation of the rhlAB promoter. Journal of Bacteriology, 185(20): 5976-83, 2003
[Winson et al., 1998]
Winson et al. Construction and analysis of luxCDABE-based plasmid sensors for investigating N-acyl homoserine lactone-mediated quorum sensing. FEMS Microbiology Letters, 163 (1998) 185-192