Composite

Part:BBa_K1073034:Design

Designed by: iGEM Team Braunschweig 2013   Group: iGEM13_Braunschweig   (2013-09-17)


Inducible ampR expression combined with LasR, RhlI and aeBlue expression cassettes


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1306
    Illegal NheI site found at 1329
    Illegal NheI site found at 2988
    Illegal NheI site found at 3011
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2830
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1683
    Illegal AgeI site found at 1880
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

no considerations.


Source

The parts for this construct were derived from the 2013 distribution kit and the iGEM Team Uppsala.

References

[Fisher et al., 1978]

Fisher et al. Kinetic studies on the inactivation of Escherichia coli RTEM β-lactamase by clavulanic acid. Biochemistry, 1978, 17 (11), pp 2180–2184

[Medina et al., 2003]

Medina G., et al., Mechanism of Pseudomonas aeruginosa RhlR transcriptional regulation of the rhlAB promoter. Journal of Bacteriology, 185(20): 5976-83, 2003

[Winson et al., 1998]

Winson et al. Construction and analysis of luxCDABE-based plasmid sensors for investigating N-acyl homoserine lactone-mediated quorum sensing. FEMS Microbiology Letters, 163 (1998) 185-192